Recipe

Synthesis Buffer (10×)

Reagent Final concentration
Tris-HCl (pH 7.4)  100 mm
dNTPs (dTTP, dATP, dCTP, and dGTP) 5 mm each
ATP   10 mm
MgCl2   50 mm
DTT   20 mm
Divide into aliquots and store at −80°C.
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  1. doi:10.1101/pdb.rec098871 Cold Spring Harb Protoc 2019: pdb.rec098871- © 2019 Cold Spring Harbor Laboratory Press
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