Recipe

WCE-LS (Whole Cell Extract-Low Salt) Buffer

20 mm Tris-HCl (pH 8.0)

70 mm KCl

1 mm EDTA

10% glycerol

0.1% IGEPAL CA-630

5 mm dithiothreitol (DTT)

1× cOmplete EDTA-Free Protease Inhibitors (Roche)

Prepare fresh before use.

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  1. doi:10.1101/pdb.rec105841 Cold Spring Harb Protoc 2019: pdb.rec105841- © 2019 Cold Spring Harbor Laboratory Press
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