Trypan Blue Stain (0.4%)
| Reagent | Amount per 100 mL of solution (w/v) | Final concentration |
|---|---|---|
| Trypan Blue | 0.4 g | 0.4% |
| PBS (1×) | 80 mL | |
| Dissolve 0.4 g of Trypan Blue in 80 mL of 1× PBS and bring to a slow boil. Cool to room temperature, and add PBS to a final volume of 100 mL. Store at ambient temperature. | ||
