Embryo Lysis Buffer (Xenopus)
| Reagent | Final concentration (1×) |
|---|---|
| Tris (pH 8.0) | 50 mm |
| NaCl | 100 mm |
| EDTA (pH 8.0) | 5 mm |
| Triton X-100 | 0.10% |
| β-glycerophosphatea | 50 mm |
| Protease Inhibitor Cocktail (PIC; Roche) | 1× |
| aAdditional phosphatase inhibitors or competitors may be required depending on the stability of the phosphorylation status of the protein of interest. | |
| Prepare fresh and store on ice. | |
