Extraction Buffer E1
| Reagent | Final concentration (1×) |
|---|---|
| HEPES-KOH (pH 7.5) | 50 mm |
| NaCl | 140 mm |
| EDTA (pH 8.0) | 1 mm |
| Glycerol | 10% |
| NP-40 | 0.5% |
| Triton X-100 | 0.25% |
| Dithiothreitol (DTT) | 1 mm |
| Protease Inhibitor Cocktail (PIC; Roche) | 1× |
| Phenylmethanesulfonyl fluoride (PMSF) | 0.2 mm |
| Prepare fresh in sterile H2O and store on ice. | |
