Recipe

Extraction Buffer E2

Reagent Final concentration (1×)
Tris (pH 8.0)  10 mm
NaCl 200 mm
EDTA (pH 8.0)  1 mm
EGTA (pH 8.0)  0.5 mm
Protease Inhibitor Cocktail (PIC; Roche)   1×
Phenylmethanesulfonyl fluoride (PMSF)  0.2 mm
Prepare fresh in sterile H2O and store on ice.
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  1. doi:10.1101/pdb.rec106567 Cold Spring Harb Protoc 2019: pdb.rec106567- © 2019 Cold Spring Harbor Laboratory Press
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