Recipe

Extraction Buffer E3

Reagent Final concentration (1×)
Tris (pH 6.8) 500 mm
NaCl 500 mm
Protease Inhibitor Cocktail (PIC; Roche)   1×
Prepare fresh in sterile H2O and store on ice.
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  1. doi:10.1101/pdb.rec106575 Cold Spring Harb Protoc 2019: pdb.rec106575- © 2019 Cold Spring Harbor Laboratory Press
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