Recipe

Proteinase K Buffer for Titer Determination (10×)

Reagent concentration Quantity (for 100 mL) Final concentration
Tris-HCl (1 m, pH 8)  1 mL 10 mm
EDTA (500 mm)  2 mL 10 mm
SDS (10%) 10 mL  1%
H2O 87 mL
Sterilize by passing through a 0.22-µm filter. Store at −20°C.
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  1. doi:10.1101/pdb.rec099523 Cold Spring Harb Protoc 2019: pdb.rec099523- © 2019 Cold Spring Harbor Laboratory Press

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