DNA Extraction Solution
| Reagent | Quantity (for 10 mL) | Final concentration |
|---|---|---|
| Deoxycholate (DOC) (10%) | 0.5 mL | 0.25% |
| Tween solution (10%) <R> | 0.9 mL | 0.45% |
| Proteinase K buffer for titer determination(10×) <R> | 2 mL | 2× |
| Proteinase K (10 mg/mL) | 0.6 mL | 0.3 mg/mL |
| H2O | 6 mL | |
| Do not store; make fresh immediately before use. Final concentrations take into account the twofold dilution incurred when adding the extraction solution to the tissue culture wells. | ||
