Recipe

Tris-Based Immunoblot Wash Buffer (TBST)

Reagent Quantity (for 1 L) Final concentration
TBS (10×)    100 mL
Tween 20 (10%, v/v; stock solution)     5 mL 0.05% (v/v)
Ultrapure H2O Up to 1000 mL
Store for up to 2–3 wk at room temperature or for up to several months at 4°C.
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  1. doi:10.1101/pdb.rec100800 Cold Spring Harb Protoc 2019: pdb.rec100800- © 2019 Cold Spring Harbor Laboratory Press
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