Tris-Buffered Saline (TBS) (10×)
| Reagent | Quantity (for 1 L) | Final concentration |
|---|---|---|
| Tris base | 24.2 g | 250 mm |
| NaCl | 80 g | 1.37 m |
| Ultrapure H2O | Up to 800 mL | |
| Adjust the pH to 7.6 using HCl, then bring the volume up to 1 L. To prepare a 1× solution, mix one part of 10× TBS with nine parts of ultrapure H2O. | ||
