DAB Substrate Solution for HRP
| Reagent | Quantity (for 10 mL) | Final concentration |
|---|---|---|
| Tris (1 m, pH 7.6) | 0.5 mL | 50 mm |
| DAB tetrahydrochloride | 6 mg | 0.6 mg/mL |
| NiCl2 (0.3%, w/v; stock solution) | 1.0 mL | 0.03% (w/v) |
| Ultrapure H2O | Up to 10 mL | |
| Dissolve all components, and filter the solution to remove any precipitate using Whatman paper filter or similar. Use the solution within 1 h. | ||
