Corrigendum: Chromatin Immunoprecipitation for Chromatin Interaction Analysis Using Paired-End-Tag (ChIA-PET) Sequencing in Tadpole Tissues
Cold Spring Harb Protoc 2018; doi: 10.1101/pdb.prot097725
In earlier versions of this protocol, the volumes of Tris-HCl and EDTA in the SDS Lysis Buffer recipe were inadvertently reversed: The volume of Tris-HCl should have been 175 µL (not 70 µL), and the volume of EDTA should have been 70 µL (not 175 µL). These volumes result in final concentrations of 50 mm and 10 mm for Tris-HCl and EDTA, respectively. The authors apologize for this error. A corrected version of the recipe is below, and the HTML version of the recipe (doi: 10.1101/pdb.rec104711) and the most recent PDF version of the protocol have been amended accordingly.
SDS Lysis Buffer
| Reagent | Amount to add (for 3.5 mL) | Final concentration (1×) |
|---|---|---|
| SDS (10%; Promega V6553) | 350 µL | 1% |
| Tris-HCl (1 m, pH 8.1) | 175 µL | 50 mm |
| EDTA (0.5 m, pH 8; Ambion AM9260G) | 70 µL | 10 mm |
| Complete Mini EDTA-free Proteinase Inhibitor Cocktail (Roche 11836170001) | 0.5 tablet | 1× |
| Phenylmethylsulfonyl fluoride (PMSF; 0.1 m in isopropanol; Fluka 93482) | 35 µL | 1 mm |
| Keep this buffer at room temperature to avoid SDS precipitation. Add protease inhibitors (Complete Mini EDTA-free Proteinase Inhibitor Cocktail and PMSF) immediately before using the buffer. | ||
