Purification of Total RNA from Mammalian Cells and Tissues
Abstract
This protocol describes the use of a monophasic lysis reagent to isolate total RNA from mammalian cells (grown as either a monolayer or in suspension) or tissues. An alternative protocol for the isolation of RNA from a small quantity of cells (102–104) or tissue (1–10 mg) is also included. In the latter case, glycogen is added to the monophasic lysis reagent and is used as a carrier to increase the recovery of RNA.
Footnotes
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From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.
