SDS Gel-Loading Buffer (5×)
| Reagent | Quantity (for 1 mL) | Final concentration |
|---|---|---|
| Tris-Cl (1 m, pH 6.8) | 0.25 mL | 250 mm |
| SDS (electrophoresis grade) | 80 mg | 8% |
| Bromophenol blue | 1 mg | 0.1% |
| Glycerol (80%, v/v) | 0.5 mL | 40% (v/v) |
| Dithiothreitol (1 m) | 0.1 mL | 100 mm |
| H2O | 0.25 mL | |
| SDS gel-loading buffer (5×) lacking DTT can be stored at room temperature. Add DTT from a 1 m stock just before the buffer is used. | ||
