Recipe

Glutathione Elution Buffer for Affinity Chromatography

Reagent Quantity (for 1 L) Final concentration
Tris-HCl (1 m, pH 8.0)  50 mL  50 mm
NaCl 29.22 g 500 mm
Reduced glutathionea  3.07 g  10 mm
Store at 4°C.
aAdd immediately before use.
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  1. doi:10.1101/pdb.rec105320 Cold Spring Harb Protoc 2020: pdb.rec105320- © 2020 Cold Spring Harbor Laboratory Press
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