| Sodium phosphate (pH 7.4) |
1.13 g of sodium phosphate monobasic, anhydrous and 5.75 g of sodium phosphate dibasic, anhydrous |
50 mm |
| NaCl |
29.22 g |
500 mm |
| Store at 4°C. |
| A nonionic detergent (0.1% Triton X-100 or 2% β-octyl glucoside) may be included in the wash buffer to reduce nonspecific
hydrophobic interactions.
|
| If the target protein contains cysteines and is an intracellular protein, reducing agents such as 1 mm DTT should be included in the wash buffer. DTT (1 m) stock solutions should be made fresh or stored as aliquots at −20°C and thawed immediately before use.
|
