IMAC Binding Buffer for Protein Purification
| Reagent | Quantity (for 1 L) | Final concentration |
|---|---|---|
| Sodium phosphate (pH 7.4) | 1.13 g of sodium phosphate monobasic, anhydrous and 5.75 g of sodium phosphate dibasic, anhydrous | 50 mm |
| NaCl | 29.22 g | 500 mm |
| Imidazole (pH 8.0) | 0.68 g | 10 mm |
-
Store at 4°C.
