EBC Lysis Buffer for ChIP
| Reagent | Volume per 100 mL of solution (v/v) | Final concentration |
|---|---|---|
| NaCl (5 m) | 2.4 mL | 120 mm |
| Nonidet P-40 (10%) | 5.0 mL | 0.5% |
| Leupeptin (10 mg/mL) | 50 µL | 5 µg/mL |
| Aprotinin (10 mg/mL) | 100 µL | 10 µg/mL |
| PMSF (0.1 m) | 1 mL | 1 mm |
| Na-vanadate (0.2 m) | 100 µL | 0.2 mm |
| NaF (1 m) | 10 mL | 100 mm |
| Tris-HCl (1 m, pH 8.0) | 5.0 mL | 50 mm |
| ddH2O | Add to 100 mL | |
| Store at 4°C. Add protease inhibitors immediately before use. | ||
