Lysis Buffer 1 for ChIP
| Reagent | Volume per 100 mL of solution (v/v) | Final concentration |
|---|---|---|
| HEPES-KOH (1 m, pH 7.5) | 5 mL | 50 mm |
| NaCl (5 m) | 2.8 mL | 140 mm |
| EDTA (0.5 m) | 0.2 mL | 1 mm |
| Glycerol (50%) | 20 mL | 10% |
| Nonidet P-40 (10%) | 5.0 mL | 0.5% |
| Triton X-100 (10%) | 2.5 mL | 0.25% |
| ddH2O | Add to 100 mL | |
| Store at 4°C. | ||
