Homogenization Buffer for Yeast
| Reagent | Quantity (for 1 L) | Final concentration |
|---|---|---|
| Sucrose | 113 g | 0.33 m |
| Tris-Cl (1 m, pH 8.0) | 300 mL | 0.3 m |
| EDTA (0.4 m, pH 8.0) | 2.5 mL | 1 mm |
| EGTA (0.4 m, pH 8.0) | 2.5 mL | 1 mm |
| Dithiothreitol (DTT) | 0.31 g | 2 mm |
| 6-Aminohexanoic acid (EACA) | 13.1 g | 100 mm |
| Store for up to 1 wk at 4°C; if stored longer, add fresh DTT before use. | ||
