Basket format for higher-throughput staining. (A) Sample basket made from a 1.7-mL microcentrifuge tube (with cap and bottom cut off) with 300-µm mesh melted to the bottom. (B) Wash setup, with color-coded baskets arranged in a 3D-printed rack within a glass staining dish. Samples share a common wash buffer. (C) Probe incubation setup, in baskets within 15-mL round-bottom culture tubes with 500 µL of probe solution. (D) Antibody incubation setup within a 48-well culture plate with 500 µL of antibody solution in each well. These incubation setups allow for each sample to experience a different probe or antibody, if desired, and minimize the total volume required.