Table 1.

Common antibodies compatible with this immunostaining procedure in stage 46 tadpoles along with relevant information

Antigen Labels Host Company Product number Dilution
β-tubulin Neurons, microtubules Mouse DSHB E7 1:100
PCNA Cells in S phase Mouse Life Technologies 133900 1:50
pHH3 Cells in M phase Rabbit Millipore 06-570 1:250
α-tubulin Spindles, microtubules Mouse DSHB 12G10 1:100
Ac-α-tubulin Cilia Mouse Sigma-Aldrich T6793 1:700
Cleaved Caspase 3 Cell death Rabbit BD Pharmingen 559565 1:250

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  1. Published in Advance April 7, 2021, doi: 10.1101/pdb.prot105635 Cold Spring Harb Protoc 2021: pdb.prot105635-
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