Recipe

Bleaching Solution for Immunofluorescence

Reagent Volume (for 100 mL) Final concentration
PBS (10×; pH 7.4) 10 mL   1×
Formamide 5 mL   5%
Peroxide (30%) 4 mL 1.2%
H2O 81 mL
Add the formamide and peroxide to the larger volume of PBS, in that order only; formamide and peroxide can become explosive if mixed directly together. This solution must be made fresh. Do not store.
| Table of Contents

This Article

  1. doi:10.1101/pdb.rec107540 Cold Spring Harb Protoc 2021: pdb.rec107540- © 2021 Cold Spring Harbor Laboratory Press
  1. » Full Text

Article Category

  1. Recipe

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Submit Updates/Comments
  2. No Updates/Comments published
  3. Alert me when Updates/Comments are published

Share

Current Issue

  1. April 2026, 2026 (4)
  1. Current Issue