Steinberg's Solution (100× Stock)
| Reagent | Final concentration |
|---|---|
| KCl | 6.7 mm |
| Ca(NO3)2 4H2O | 3.4 mm |
| MgSO4 7H2O | 8.3 mm |
| NaCl | 580 mm |
| HEPES | 499.37 mm |
| Prepare the 100× stock solution with deionized H2O. Adjust pH to 7.5 using 10 n NaOH. Store. at 4°C. To prepare a 1× working solution, dilute 1/100 in deionized H2O (i.e., 1 part 100× stock + 99 parts H2O). | |
