Plating Culture Medium for Neural Tube Explants

Recipe

Reagent	Amount to add	Final concentration
Fetal bovine serum (Gibco 10438018)	20 µL	1%
Penicillin-Streptomycin (Sigma-Aldrich P4333)	20 µL	1%
BDNF (100 µg/mL; Sigma-Aldrich B3795; optional^a)	0.5 µL	25 ng/mL
NT3 (25 µg/mL; Sigma-Aldrich SRP3128; optional^a)	2 µL	25 ng/mL
Add culture media base to a final volume of 2 mL (per 35-mm plate). Prepare plating culture medium fresh and maintain at 4°C.
^aAxon outgrowth is greatly enhanced by addition of BDNF and NT3, but these may be omitted for guidance assays if cues will be added later.