Developing Solution (for AP)
| Reagent | Quantity |
|---|---|
| Diethanolamine (DEA) substrate buffer (5× concentrate) (Thermo Scientific 34064) | 4 mL |
| 4-Nitrophenyl phosphate (pNPP) disodium salt hexahydrate tablets (Sigma-Aldrich N2765) | 20 mg |
| Thirty minutes before developing the plate, dissolve one 20-mg pNPP tablet in 20 mL of 1× diethanolamine (DEA) solution (prepared by combining 16 mL H2O with 4 mL 5× DEA). | |
