Recipe

PBTr

Reagent Quantity Final concentration
Triton X-100 (Sigma-Aldrich SLBT2255) 70–200 µL 0.7%–2%
10× PBS (Gibco 2000522) 1 mL 1×

  • Determine the appropriate amount of Triton X-100 empirically. Add double-distilled H2O to 10 mL. Prepare fresh before use.

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  1. doi:10.1101/pdb.rec107878 Cold Spring Harb Protoc 2022: pdb.rec107878- © 2022 Cold Spring Harbor Laboratory Press
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