Hybridoma Screening by Antibody Capture: Detection of Cell-Surface Binding by Immunofluorescence
Abstract
If the antigen of interest is a cell-surface protein, immunofluorescence can be used to identify hybridomas secreting monoclonal antibodies to these proteins. They can be stained on microscope chamber slides, flat-bottomed cell culture plates (96, 48, or 24 well), or imaging plates (96 well). This protocol uses 96-well imaging plates.
Footnotes
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From the Antibodies collection, edited by Edward A. Greenfield.
