Recipe

Lysis Buffer for Tadpoles

50 mm Tris (pH 8.8)

1 mm EDTA

0.5% Tween 20

200 µg/mL proteinase K (PCR grade; RPROTK-RO ROCHE; Sigma-Aldrich)

Store at room temperature. Add proteinase K immediately prior to use.

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  1. doi:10.1101/pdb.rec107719 Cold Spring Harb Protoc 2022: pdb.rec107719- © 2022 Cold Spring Harbor Laboratory Press
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