Recipe

High-Salt Solution (10× Stock)

280 g NaCl

7.2 g KCl

14.1 g CaCl2

9.7 g MgCl2

17.12 g HEPES

Bring to 4 L with sterile water. Adjust the pH to 7.6 with NaOH. Filter-sterilize and store at room temperature.

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  1. doi:10.1101/pdb.rec107720 Cold Spring Harb Protoc 2022: pdb.rec107720- © 2022 Cold Spring Harbor Laboratory Press
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