Protocol

Golgi Silver Staining of Mosquito Central Neuropils

  1. Sharon Rose Hill
  1. Disease Vector Group, Unit of Chemical Ecology, Department of Plant Protection Biology, Swedish University of Agricultural Sciences, 23422 Alnarp, Sweden
  1. ↵1Correspondence: rickard.ignell{at}slu.se

Abstract

The Golgi silver staining procedure relies on three interdependent stages: fixation, chromation, and metal impregnation. Each of these stages can be modified. This protocol describes a method demonstrated to stain neurons within the mosquito central nervous system. The resulting preparations are stable at room temperature.

Footnotes

  • From the Mosquitoes collection, edited by Laura B. Duvall and Benjamin J. Matthews.

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This Article

  1. Published in Advance May 13, 2022, doi: 10.1101/pdb.prot107840 Cold Spring Harb Protoc 2022: pdb.prot107840- © 2022 Cold Spring Harbor Laboratory Press
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