Chitinase-Chymotrypsin Dimethyl Sulfoxide Buffer (CCD)
| Reagent | Amount | Final concentration |
|---|---|---|
| HEPES larval buffer (HLB) | 10 mL | |
| Chitinase (Sigma-Aldrich C6137) | 50 U | 5 U/mL |
| Chymotrypsin (Sigma-Aldrich CHY5S-10VL) | 1000 U | 100 U/mL |
| Dimethyl sulfoxide (DMSO) (e.g., Sigma-Aldrich 472301) | 100 µL | 1% |
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Add HLB to chitinase. Resuspend chymotrypsin (five vials of 200 U/vial) in HLB/chitinase solution and DMSO. Aliquot and store at −20°C.
