Fixative Solution for Immunostaining
| Reagent | Amount |
|---|---|
| Phosphate-buffered saline (10×) (e.g., Thermo Fisher AM9625) | 1 mL |
| Deionized H2O | 7 mL |
| Paraformaldehyde (20%) | 2 mL |
| Triton X-100 (10%) (e.g., Sigma-Aldrich 93443) | 30 µL |
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Prepare fresh.
