Gel Loading Buffer (10×)
| Reagent | Quantity for 10 mL |
|---|---|
| Orange G (Fisher Scientific AC416550100) | 100 mg |
| Tris-EDTA solution (10×, pH 7.4; Fisher Scientific BP2477100) | 5 mL |
| Glycerol (80% v/v) | 5 mL |
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1. Add Orange G to 10× Tris-EDTA solution (pH 7.4).
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2. Vortex to dissolve.
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3. Add 80% glycerol.
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4. Vortex to mix.
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5. Store at room temperature.
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The buffer is stable for up to 5 yr at room temperature.
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