Molten Agarose
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1. Add either 0.8 or 1.5 g of agarose (Fisher Scientific BP160) to 100 mL of 1× TAE electrophoresis running buffer in a 250-mL glass bottle to make a 0.8% or 1.5% solution, respectively.
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2. Microwave until boiling starts, and then carefully swirl the bottle.
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3. Continue microwaving at reduced power with occasional swirling until the agarose is fully dissolved.
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4. Cool to 60°C before use.
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The molten 0.8% agarose can be stored for up to 1 wk at 60°C, but the 1.5% agarose should be used fresh. Single-use aliquots of either can be stored for up to 6 mo at 4°C, and then microwaved to remelt.
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