Lysis Buffer for Killifish Microbiota Sequencing
80 mm EDTA (Life Technologies AM9261)
200 mm Tris-Cl (pH 8.0; Tris base, Sigma-Aldrich T3069-1L)
100 mm NaCl
1× Phosphate-buffered saline (pH 7.4; PBS, Life Technologies AM9625)
Filter-sterilize and store for up to 12 mo at room temperature. Dilute with autoclaved MilliQ-purified H2O as appropriate.
