Tris(hydroxymethyl)aminomethane Buffered Saline with Triton X-100 (TBST; 10×)
0.1 m Tris
15 m NaCl
0.015 m Triton X-100
Prepare in milliQ-purified H2O and adjust the pH to 7.6 with 1 m HCl. Check and adjust the pH before bringing the working solution up to the final dilution volume. Protect from light and store for up to 1 yr at room temperature.
