Cutting Buffer (10×)
80 mm Na2HPO4.2H2O
20 mm KH2PO4
Prepare in distilled, deionized, milliQ-purified H2O and adjust pH to 7.4 with 5 m NaOH. Make sure to check and adjust the pH before bringing the stock solution up to the final dilution volume. Store for up to 1 yr at 4°C.
