Tricaine Solution
Dissolve ethyl 3-aminobenzoate methanesulfonate (tricaine; Sigma-Aldrich E10521) in tank water to achieve a concentration of 0.5 mg/mL.
Store for no longer than 2–3 mo at 4°C. We note that our recommendation is largely based on the fact that the solution is usually used up by then rather than because we have observed any loss of potency after that period.
