Recipe

Hatching Solution

Reagent Quantity
Humic acid (commercially available) 1 g
Distilled/deionized/reverse osmosis H2O 1 L

  • Do not autoclave. Store at 4°C. In our experience, the hatching solution still works with high efficiency after 6 mo at 4°C.

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  1. doi:10.1101/pdb.rec108058 Cold Spring Harb Protoc 2023: pdb.rec108058- © 2023 Cold Spring Harbor Laboratory Press
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