Protocol

Electrophysiological Measurements of Compound Action Potential Responses from the Antennal Nerve in Response to Stimulation

  1. Joerg T. Albert3,4,5
  1. 1Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan
  2. 2Institute for Advanced Research, Nagoya University, Nagoya 464-8602, Japan
  3. 3Ear Institute, University College London, London WC1X 8EE, United Kingdom
  4. 4The Francis Crick Institute, London NW1 1AT, United Kingdom
  1. 5Correspondence: joerg.albert{at}ucl.ac.uk

Abstract

Electrophysiological recordings taken from the antennal nerve can provide essential information on the general auditory condition of the mosquito tested. Furthermore, electrophysiological recordings provide detailed information on what types of stimulation induce the largest nerve responses. When these are used in conjunction with a vibrometer to measure the corresponding movement of the antennal ear during stimulation, a comprehensive overview of hearing function can be obtained. This protocol can be applied to male and female adults from any mosquito strain and can be scaled relative to available resources.

Footnotes

  • From the Mosquitoes collection, edited by Laura B. Duvall and Benjamin J. Matthews.

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  1. Cold Spring Harb Protoc 2023: pdb.prot108010- © 2023 Cold Spring Harbor Laboratory Press
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