1× XB
1× XB salts, dilute from Extract buffer (XB) salts (20×)
50 mm sucrose (e.g., Sigma-Aldrich SX1075; dilute from a 1.5 m stock solution that has been filter-sterilized and stored at −20°C)
10 mm HEPES (e.g., Sigma-Aldrich H3375; dilute from a 1 m, pH 7.7 stock solution that has been filter-sterilized and stored at −20°C)
Freshly prepare 1 L and adjust pH to 7.7 with KOH.
