Recipe

Artificial Meal

Reagent Final concentration
NaCl (500 mm, stored at room temperature) 110 mm
NaHCO3 (100 mm, stored at room temperature) 20 mm
ATP (20 mm in 25 mm NaHCO3, stored at −20°C) 1 mm
  • Prepare the artificial meal fresh before each experiment, and use it within 1 d. This formulation was used in Jové et al. (2020) and Hol et al. (2020). See Duvall et al. (2019). for artificial meal formulations that include a variety of common proteins present in blood.

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  1. doi:10.1101/pdb.rec108178 Cold Spring Harb Protoc 2023: pdb.rec108178- © 2023 Cold Spring Harbor Laboratory Press

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