Recipe

Tris–Sucrose–Magnesium Chloride (TSM) Buffer

Tris HCl (50 mm, pH 7.5)

Sucrose (0.5 m)

MgCl2 (10 mm)

Filter-sterilize or autoclave using a short program. Store at 4°C.

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  1. doi:10.1101/pdb.rec108123 Cold Spring Harb Protoc 2023: pdb.rec108123- © 2023 Cold Spring Harbor Laboratory Press
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