Figure 1.
Figure 1.

Schematic overview of the recombineering procedure. (A) Relevant steps involved. Regions of sequence homology between the donor fragment and chromosomal DNA are shown in salmon red. (B) Insertion frame requirements for reading frame maintenance following kan cassette excision.

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  1. Published in Advance February 22, 2023, doi: 10.1101/pdb.prot107856 Cold Spring Harb Protoc 2023: pdb.prot107856-
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