Phosphate-Buffered Saline with 1% BSA (PBSB)
-
1. Combine the following salt components in distilled H2O.
| Reagent | Final concentration |
|---|---|
| NaCl | 150 mm |
| Na2HPO4 | 8 mm |
| NaH2PO4 | 2 mm |
-
2. Adjust salt components and distilled H2O to reach pH 7.4 by using NaOH or HCl for a final volume of 1 L.
-
3. Autoclave the solution at 15 PSI for 20 min at 121°C on a liquid cycle. Verify the pH again.
-
4. Dissolve BSA (e.g., SaveenWerner B2001-500) to final concentration of 1% (w/v). Adjust the volume to 1 L and sterilize with a 0.2-µm filter. Prepare fresh before use. Keep at 4°C.
