Sucrose (0.5 m)/Glycerol (10%)
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1. Mix 57.5 mL of 85% (v/v) glycerol in H2O with 500 mL of distilled H2O.
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2. Autoclave at 15 PSI for 20 min at 121°C.
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3. Add 85.6 g of sucrose and sterilize with a 0.2-µm filter.
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Store for up to months at room temperature or 4°C.
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