Recipe

RNA Hybridization Solution

Reagent Final concentration
Formaldehyde 50% (v/v)
Saline sodium citrate (SSC), 20× (Invitrogen 15557044)
Heparin 0.1 mg/mL
Sonicated salmon sperm DNA 0.1 mg/mL
Tween-20 0.1% (v/v)
  • Store for up to 1 yr at −20°C.

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  1. doi:10.1101/pdb.rec108486 Cold Spring Harb Protoc 2024: pdb.rec108486- © 2024 Cold Spring Harbor Laboratory Press

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