Recipe

Separation/Resolving Gel Mixture (12%)

Tris-HCl (1.5 m, pH 8.8) 2.5 mL
Double distilled H2O 4.29 mL
Acrylamide/Bis solution 29:1 (30%; Bio-Rad) 3 mL
SDS, 10% (w/v) 0.1 mL
Ammonium persulfate, 10% (w/v) 0.1 mL
Tetramethylethylenediamine
 (TEMED; Bio-Rad Laboratories 161-0801) 0.01 mL

Freshly prepare. Add TEMED last, immediately before pouring the gel solution into the gel casting apparatus.

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  1. doi:10.1101/pdb.rec108495 Cold Spring Harb Protoc 2024: pdb.rec108495- © 2024 Cold Spring Harbor Laboratory Press
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